[Verified Answer]: Ideally what volume of clearing agent…

Ideally what volume of clearing agent should be used? a) 2 – 5 X that of tissue b) 5 – 10 X that of tissue c) 15 – 20 X that of tissue d) 25 – 50 X that of tissue e) 50 – 100 X that of tissue 8) In the H+E stain, wax is removed with a) 70% alcohol b) 100% alcohol c) formalin d) water e) xylol 9) Choose the correct order for staining tissue sections with hematoxylin a) bluing, clearing, differentiation, hydration, staining b) clearing, dehydration, staining, differentiation, bluing c) clearing, hydration, staining, differentiation, bluing d) fixation, dehydration, clearing, infiltration, embedding e) tissue selection, fixation, decalcification, neutralization, washing 10) After grossing, tissue specimens are chosen for processing and placed a) in embedding molds b) in tissue cassettes c) on microscope slides d) under coverslips 11) Decalcification is sometimes necessary because calcium will a) contaminate the xylene b) interfere with sectioning c) prevent penetration with wax d) prevent the blood in the tissue from clotting 12) Which of the following is a clearing agent? a) acetic acid b) alcohol c) formalin d) nitric acid e) xylene 13) Dehydration requires a) ascending grades of alcohol b) ascending grades of xylene c) descending grades of alcohol d) descending grades of NeoClear e) descending grades of xylene 14) Routine mounting of stained tissue sections involves coverslipping the section with a) cedarwood oil b) iodine c) immersion oil d) isotonic saline e) permanent mounting media 15) STAT surgical specimens are processed by cutting a) frozen sections with a cryostat b) frozen sections with a scalpel c) paraffin sections with a cryostat d) paraffin sections with a microtome e) paraffin sections with a scalpel 16) When embedding, correct tissue orientation includes most of the following. Which one is NOT correct? Tissues should be placed a) at a consistent depth b) close to the bottom of the mold c) close together in a line d) with edges parallel to mold edge 17) The process for assigning unique identification numbers to laboratory specimens is described as a) accessioning b) grossing c) labeling d) numbering